Design and synthesis of novel photoinduced electron transfer-based hybridization probes

Publication date: Available online 6 May 2017
Source:Bioorganic & Medicinal Chemistry
Author(s): Chika Yokoyama, Kosuke Nakamoto, Yoshihito Ueno
Photoinduced electron transfer (PeT)-based hybridization probe is a linear and quencher-free oligonucleotide (ON) probe for DNA or RNA detection. In this report, we designed and synthesized novel adenosine analogues for PeT-based hybridization probe. In particular, the analogue containing a piperazinomethyl moiety showed effective quenching property under physiological conditions. When the probe containing the analogue was hybridized with a complementary DNA or RNA, the fluorescence increased 3- or 4-fold, respectively, compared to the single-stranded state.

Graphical abstract

http://ift.tt/2pmHpjf