Epithelial Histone Deacetylase 3 Instructs Intestinal Immunity by Coordinating Local Lymphocyte Activation

Publication date: 9 May 2017
Source:Cell Reports, Volume 19, Issue 6
Author(s): Nazanin Navabi, Jordan Whitt, Shu-en Wu, Vivienne Woo, Jessica Moncivaiz, Michael B. Jordan, Bruce A. Vallance, Sing Sing Way, Theresa Alenghat
Mucosal tissues are constantly in direct contact with diverse beneficial and pathogenic microbes, highlighting the need for orchestrating complex microbial signals to sustain effective host defense. Here, we show an essential role for intestinal epithelial cell expression of histone deacetylase 3 (HDAC3) in responding to pathogenic microbes and activating protective innate immunity. Mice lacking HDAC3 in intestinal epithelial cells were more susceptible to Citrobacter rodentium when under tonic stimulation by the commensal microbiota. This impaired host defense reflected significantly decreased IFNγ production by intraepithelial CD8⁺ T cells early during infection. Further, HDAC3 was necessary for infection-induced epithelial expression of the IFNγ-inducing factor IL-18, and administration of IL-18 restored IFNγ activity to resident CD8⁺ T cells and reduced infection. Thus, HDAC3 mediates communication between intestinal epithelial cells and resident lymphocytes, revealing that epithelial priming by an epigenetic modifier may direct mucosal regulation of host defense against pathogenic microbes.

Graphical abstract

Teaser

Understanding regulation of coordinated mucosal immune responses to diverse signals from the microbiota and pathogenic microbes remains a challenge. Navabi et al. identify a role for epithelial histone deacetylase 3 in directing innate antibacterial immunity by resident lymphocytes during enteric infection.


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