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Τρίτη 16 Μαΐου 2017

The INO80 Complex Removes H2A.Z to Promote Presynaptic Filament Formation during Homologous Recombination

Publication date: 16 May 2017
Source:Cell Reports, Volume 19, Issue 7
Author(s): Claudio A. Lademann, Jörg Renkawitz, Boris Pfander, Stefan Jentsch
The INO80 complex (INO80-C) is an evolutionarily conserved nucleosome remodeler that acts in transcription, replication, and genome stability. It is required for resistance against genotoxic agents and is involved in the repair of DNA double-strand breaks (DSBs) by homologous recombination (HR). However, the causes of the HR defect in INO80-C mutant cells are controversial. Here, we unite previous findings using a system to study HR with high spatial resolution in budding yeast. We find that INO80-C has at least two distinct functions during HR—DNA end resection and presynaptic filament formation. Importantly, the second function is linked to the histone variant H2A.Z. In the absence of H2A.Z, presynaptic filament formation and HR are restored in INO80-C-deficient mutants, suggesting that presynaptic filament formation is the crucial INO80-C function during HR.

Graphical abstract

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Teaser

The chromatin remodeler INO80-C is required for efficient DSB repair. Lademann et al. find that INO80-C has a dual role in homologous recombination, functioning during DNA end resection and Rad51 filament formation. Specifically, the second function is critical for DNA repair and is mechanistically promoted by a turnover of the histone variant H2A.Z.


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