Siderophore coated magnetic iron nanoparticles: Rational designing of water soluble nanobiosensor for visualizing Al3+ in live organism

Publication date: 15 November 2017
Source:Biosensors and Bioelectronics, Volume 97
Author(s): M. Raju, Sakshi Srivastava, Ratish R. Nair, Ishan H. Raval, Soumya Haldar, Pabitra B. Chatterjee
This article aims to establish the judicious use of iron-binding chemistry of microbial chelators in order to functionalize the surface of iron nanoparticles to develop non-toxic nanobiosensor. Anchoring a simple siderophore 2,3-dihydroxybenzoylglycine (H3L), which bears catechol and carboxyl functionalities in tandem, on to the surface of Fe3O4 nanoparticles has developed a unique nanobiosensor HL-FeNPs which showed highly selective and sensitive detection of Al³⁺ in 100% water at physiological pH. The biosensor HL-FeNPs, with 20nM limit of detection, behaves reversibly and instantly. In-vivo bio-imaging in live brine shrimp Artemia confirmed that HL-FeNPs could be used as fluorescent biomarker for Al³⁺ in live whole organisms. Magnetic nature of the nanosensor enabled HL-FeNPs to remove excess Al³⁺ by using external magnet. To our knowledge, the possibility of microbial chelator in the practical development of Al³⁺ selective nanobiosensor is unprecedented.

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