A single dose of the novel chimeric subunit vaccine E2-CD154 confers early full protection against classical swine fever virus

Publication date: Available online 5 July 2017
Source:Vaccine
Author(s): Marisela Suárez, Yusmel Sordo, Yanet Prieto, María P. Rodríguez, Lídice Méndez, Elsa M. Rodríguez, Alina Rodríguez-Mallon, Elianet Lorenzo, Elaine Santana, Nemecio González, Paula Naranjo, María Teresa Frías, Yamila Carpio, Mario Pablo Estrada
Classical swine fever is an economically important, highly contagious disease of swine worldwide. Subunit vaccines are a suitable alternative for the control of classical swine fever. However, such vaccines have as the main drawback the relatively long period of time required to induce a protective response, which hampers their use under outbreak conditions. In this work, a lentivirus-based gene delivery system is used to obtain a stable recombinant HEK 293 cell line for the expression of E2-CSFV antigen fused to porcine CD154 as immunostimulant molecule. The E2-CD154 chimeric protein was secreted into the medium by HEK293 cells in a concentration around 50mg/L in suspension culture conditions using spinner bottles. The E2-CD154 immunized animals were able to overcome the challenge with a high virulent CSF virus strain performed 7days after a unique dose of the vaccine without clinical manifestations of the disease. Specific anti-CSFV neutralizing antibodies and IFN-γ were induced 8days after challenge equivalent to 14days post-vaccination. The present work constitutes the first report of a subunit vaccine able to confer complete protection by the end of the first week after a single vaccination. These results suggest that the E2-CD154 antigen could be potentially used under outbreak conditions to stop CSFV spread and for eradication programs in CSF enzootic areas.



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