Antrodia camphorata inhibits metastasis and epithelial-to-mesenchymal transition via the modulation of claudin-1 and Wnt/β-catenin signaling pathways in human colon cancer cells

Publication date: 17 August 2017
Source:Journal of Ethnopharmacology, Volume 208
Author(s): You-Cheng Hseu, Yu-Hsien Chao, Kai-Yuan Lin, Tzong-Der Way, Hui-Yi Lin, Varadharajan Thiyagarajan, Hsin-Ling Yang
Ethnopharmacological relevanceAntrodia camphorata (AC) is a well known traditional Chinese medicinal mushroom in Taiwan, has been used to treat various diseases including cancer.Materials and methodsIn this study, we investigated the anti-metastatic and anti-EMT properties of a fermented culture broth of AC in human colon SW480^claudin−1- and metastatic SW620^claudin−1+ cancer cells in vitro.ResultsAC down-regulates claudin-1 and inhibits the proliferation and colony-formation abilities of both SW620^claudin−1+ and SW480^claudin−1- cells. In highly metastatic SW620^claudin−1+ cells, non-cytotoxic concentrations of AC significantly inhibited migration/invasion, accompanied by the down-regulation of MMP-2 and MMP-9 proteins. AC decreased nuclear translocation of Wnt/β-catenin through a GSK3β-dependent pathway. AC consistently inhibited EMT by up-regulating the epithelial and downregulating the mesenchymal marker proteins. In SW480^claudin−1- cells, AC suppressed migration/invasion potentially through the inhibition of the PI3K/AKT/NFκB signaling pathways without altering the expression levels of β-catenin and GSK3β proteins.ConclusionAltogether, this study demonstrates the anti-metastatic and anti-EMT activities of AC, which may contribute to the development of a chemopreventive agent for colon cancer.

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