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Τετάρτη 26 Ιουλίου 2017

iTRAQ proteomic analysis of the interactions between Bombyx mori nuclear polyhedrosis virus and silkworm

Publication date: Available online 26 July 2017
Source:Journal of Proteomics
Author(s): Wei-tao Dong, Long-fei Xiao, Jun-jie Hu, Xin-xu Zhao, Ji-xing Liu, Yong Zhang
The silkworm hemolymph is an important defense system against bacteria and viruses. In this study, silkworms were infected with Bombyx mori nuclear polyhedrosis virus to investigate the subsequent immune response at the protein level. Proteomes were analyzed before and after infection using isobaric tags for relative and absolute quantitation and LC-MS. A total of 456 differentially expressed proteins were identified, of which 179 were upregulated and 193 were downregulated. Changes in expression were validated by western blot for several proteins. Eleven of the differentially expressed proteins were involved in immunity. For example, modular serine protease and cecropin, which were downregulated, facilitate Toll and Imd signaling, while autophagy-related protein 3, which was upregulated, protects cells against oxidative damage. Collectively, the data highlight the unique interactions of baculovirus with the silkworm immune system.Biological significanceThis is the first time isobaric tags for relative and absolute quantitation were used to analyze B. mori proteins mobilized against B. mori nuclear polyhedrosis virus, and to investigate the immunity-associated proteome in B. mori. The results are a significant step towards a deeper understanding of immunoregulation in B. mori.SignificanceThis is the first time isobaric tags for relative and absolute quantitation were used to analyze B. mori proteins mobilized against B. mori nuclear polyhedrosis virus, and to investigate the immunity-associated proteome in B. mori. The results are a significant step towards a deeper understanding of immunoregulation in B. mori.

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