Photodynamic therapy with high-power LED mediated by erythrosine eliminates Enterococcus faecalis in planktonic forms

Publication date: Available online 25 July 2017
Source:Photodiagnosis and Photodynamic Therapy
Author(s): Alberto Sabin Moura Borba, Sângela Maria da Silva Pereira, Mellyna Cavalcante Mendes Borba, Marco Aurélio Benini Paschoal, Rudys Rodolfo de Jesus Tavarez, Claudia de Castro Rizzi, Meire Coelho Ferreira, Etevaldo Matos Maia Filho
BackgroundThe failure of endodontic treatment is linked to the presence of microorganisms, particularly Enterococcus faecalis, in the root canals. This study evaluated the effectiveness of photodynamic therapy (PDT) using erythrosine irradiated by a high-power curing light on a planktonic suspension culture of E. faecalis.MethodsBacterial suspensions of E. faecalis were adjusted and then mixed in a 1:1 proportion, in triplicate, in treatment groups by varying the length of irradiation time (120 and 240seconds) and the molarity of the erythrosine (5 and 10μM). In order to verify the post-treatment bactericidal effect, a count of the viable bacteria was performed (CFUmL⁻¹) and transformed into Log10 CFU. The one-way ANOVA with Tukey post-hoc test was applied to check for differences between the groups.ResultsThe bacteria were completely eradicated in the groups that used PDT with 5μM 240s, 10μM 120s and 10μM 240s (p <0.001). The effect of the PDT 5μM 120s group was significant (p<0.05) in comparison with the groups using only light or only erythrosine. Positive control (exposure to 2.5% NaClO for 120 and 240s) completely eradicated E. faecalis. The negative control (PBS) did not alter the quantities of E. faecalis CFU with 9.605 Log10 CFU at 120s and 9.621 Log10 CFU at 240s.ConclusionPDT with erythrosine in a concentration of 10μM and high-power LED is capable of totally eliminating E. faecalis in planktonic suspension.



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