αE-Catenin Is a Positive Regulator of Pancreatic Islet Cell Lineage Differentiation

Publication date: 8 August 2017
Source:Cell Reports, Volume 20, Issue 6
Author(s): Antonio J. Jimenez-Caliani, Rudolf Pillich, Wendy Yang, Giuseppe R. Diaferia, Paolo Meda, Laura Crisa, Vincenzo Cirulli
The development and function of epithelia depend on the establishment and maintenance of cell-cell adhesion and intercellular junctions, which operate as mechanosensor hubs for the transduction of biochemical signals regulating cell proliferation, differentiation, survival, and regeneration. Here, we show that αE-catenin, a key component of adherens junctions, functions as a positive regulator of pancreatic islet cell lineage differentiation by repressing the sonic hedgehog pathway (SHH). Thus, deletion of αE-catenin in multipotent pancreatic progenitors resulted in (1) loss of adherens junctions, (2) constitutive activation of SHH, (3) decrease in islet cell lineage differentiation, and (4) accumulation of immature Sox9⁺ progenitors. Pharmacological blockade of SHH signaling in pancreatic organ cultures and in vivo rescued this defect, allowing αE-catenin-null Sox9⁺ pancreatic progenitors to differentiate into endocrine cells. The results uncover crucial functions of αE-catenin in pancreatic islet development and harbor significant implications for the design of β cell replacement and regeneration therapies in diabetes.

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Teaser

Jimenez-Caliani et al. examine a regulatory function for αE-catenin in the endocrine differentiation of pancreatic progenitors. Ablation of αE-catenin in multipotent Pdx1⁺ progenitors disrupts cell-cell adhesion and leads to constitutive activation of SHH signaling that precludes endocrine differentiation and leads to the accumulation of proliferating Sox9⁺ cells. Pharmacological blockade of SHH rescues the competency of αE-catenin^nullSox9⁺ progenitors to acquire an endocrine phenotype.


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