Data for automated, high-throughput microscopy analysis of intracellular bacterial colonies using spot detection

Publication date: October 2017
Source:Data in Brief, Volume 14
Author(s): Christina L. Ernstsen, Frédéric H. Login, Helene H. Jensen, Rikke Nørregaard, Jakob Møller-Jensen, Lene N. Nejsum
Quantification of intracellular bacterial colonies is useful in strategies directed against bacterial attachment, subsequent cellular invasion and intracellular proliferation. An automated, high-throughput microscopy-method was established to quantify the number and size of intracellular bacterial colonies in infected host cells (Detection and quantification of intracellular bacterial colonies by automated, high-throughput microscopy, Ernstsen et al., 2017 [1]). The infected cells were imaged with a 10× objective and number of intracellular bacterial colonies, their size distribution and the number of cell nuclei were automatically quantified using a spot detection-tool. The spot detection-output was exported to Excel, where data analysis was performed. In this article, micrographs and spot detection data are made available to facilitate implementation of the method.



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