Key elements for designing and performing a CRISPR/Cas9-based genetic screen

Publication date: Available online 22 September 2017
Source:Journal of Genetics and Genomics
Author(s): Wanjing Shang, Fei Wang, Gaofeng Fan, Haopeng Wang
Reverse genetic screens are invaluable for uncovering gene functions, but are traditionally hampered by some technical limitations. Over the past few years, since the advent of the revolutionary CRISPR/Cas9 technology, its power in genome editing has been harnessed to overcome the traditional limitations in reverse genetic screens, with successes in various biological contexts. Here, we outline these CRISPR/Cas9-based screens, provide guidance on the design of effective screens and discuss the potential future directions of development of this field.



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