Semisynthesis of autophagy protein LC3 conjugates

Publication date: 15 September 2017
Source:Bioorganic & Medicinal Chemistry, Volume 25, Issue 18
Author(s): Aimin Yang, Inken Hacheney, Yao-Wen Wu
Autophagy is a conserved catabolic process involved in the elimination of proteins, organelles and pathogens. Autophagosome formation is the key process in autophagy. Lipidated Atg8/LC3 proteins that are conjugated to phosphatidylethanolamine (PE) play a key role in autophagosome biogenesis. To understand the function of Atg8/LC3-PE in autophagosome formation and host-pathogen interaction requires preparation and structural manipulation of lipidated Atg8/LC3 proteins. Herein, we report the semisynthesis of LC3 proteins and mutants with modifications of different PE fragments or lipids using native chemical ligation and aminolysis approaches.

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