Electrochemical synthesis of silver nanoclusters on electrochemiluminescent resonance energy transfer amplification platform for Apo-A1 detection

Publication date: 1 May 2018
Source:Talanta, Volume 181
Author(s): Ying Zhou, Yanqing Yu, Yaqin Chai, Ruo Yuan
Herein, a novel electrochemiluminescence resonance energy transfer (ERET) strategy between in situ electrogenerated silver nanoclusters (AgNCs) as the donor and Ru(bpy)2(5-NH2-1,10-phen)Cl2 (Ru(II)) as the acceptor was reported, which was applied for the construction of a ultrasensitive immunosensor for the determination of apolipoprotein-A1 (Apo-A1). Notably, the Ag NCs were in suit electro-reducted on glassy carbon electrode (GCE) based on a simple, fast and controllable electrochemical technique, which acted as not only biocompatible ECL emitters, but also protein immobilization platform via Ag–N or Ag–S bond. Subsequently, the acceptor probe was constructed by modifying Ru(II) and secondary antibody on the nanocarriers of carboxyl-functionalized MWCNTs. Based on the sandwiched immunoassay method, the ECL signals declining at 449nm (Ag NCs) and the increasing at 650nm (Ru(II)) both reflect the amount of Apo-A1. By measuring the ratio of ECL650nm/ECL449nm, we could accurately quantify the concentration of Apo-A1 in range from 1.0pg/mL to 1.0ng/mL and limit of detection down to 0.33pg/mL, which opened up a new research direction for ultrasensitive ECL bioanalysis based metal NCs.

Graphical abstract

http://ift.tt/2E6a4jk