Quantitative Operating Principles of Yeast Metabolism during Adaptation to Heat Stress

Publication date: 27 February 2018
Source:Cell Reports, Volume 22, Issue 9
Author(s): Tania Pereira, Ester Vilaprinyo, Gemma Belli, Enric Herrero, Baldiri Salvado, Albert Sorribas, Gisela Altés, Rui Alves
Microorganisms evolved adaptive responses to survive stressful challenges in ever-changing environments. Understanding the relationships between the physiological/metabolic adjustments allowing cellular stress adaptation and gene expression changes being used by organisms to achieve such adjustments may significantly impact our ability to understand and/or guide evolution. Here, we studied those relationships during adaptation to various stress challenges in Saccharomyces cerevisiae, focusing on heat stress responses. We combined dozens of independent experiments measuring whole-genome gene expression changes during stress responses with a simplified kinetic model of central metabolism. We identified alternative quantitative ranges for a set of physiological variables in the model (production of ATP, trehalose, NADH, etc.) that are specific for adaptation to either heat stress or desiccation/rehydration. Our approach is scalable to other adaptive responses and could assist in developing biotechnological applications to manipulate cells for medical, biotechnological, or synthetic biology purposes.

Graphical abstract

Teaser

Evolution selects coordinated adaptive changes in gene expression and metabolism that ensure survival to stress challenges. Pereira et al. identify quantitative ranges for those changes in a set of genes and physiological variables (production of ATP, trehalose, NADH, etc.) that are specific for adaptation to heat stress, desiccation/rehydration, or pH.


http://ift.tt/2GOPPrW