Data on the role of iba57p in free Fe2+ release and O2∙− generation in Saccharomyces cerevisiae

Publication date: June 2018
Source:Data in Brief, Volume 18
Author(s): Mauricio Gomez-Gallardo, Luis A. Sánchez, Alma L. Díaz-Pérez, Christian Cortés-Rojo, Jesús Campos-García
The related study has confirmed that in Saccharomyces cerevisiae, iba57 protein participates in maturation of the [2Fe–2S] cluster into the Rieske protein, which plays important roles in the conformation and functionality of mitochondrial supercomplexes III/IV in the electron transport chain (Sánchez et al., 2018) [1]. We determined in S. cerevisiae the effects of mutation in the IBA57 gene on reactive oxygen species (ROS) and iron homeostasis. Flow cytometry and confocal microscopy analyses showed an increased generation of ROS, correlated with free Fe²⁺ release in the IBA57 mutant yeast. Data obtained support that a dysfunction in the Rieske protein has close relationship between ROS generation and free Fe²⁺ content, and which is possible that free Fe²⁺ release mainly proceeds from [Fe–S] cluster-containing proteins.



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