Neonatal Subventricular Zone Neural Stem Cells Release Extracellular Vesicles that Act as a Microglial Morphogen

Publication date: 3 April 2018
Source:Cell Reports, Volume 23, Issue 1
Author(s): Mary C. Morton, Victoria N. Neckles, Caitlin M. Seluzicki, Jennie C. Holmberg, David M. Feliciano
Subventricular zone (SVZ) neural stem cells (NSCs) are the cornerstone of the perinatal neurogenic niche. Microglia are immune cells of the nervous system that are enriched in the neonatal SVZ. Although microglia regulate NSCs, the extent to which this interaction is bi-directional is unclear. Extracellular vesicles (EVs) are cell-derived particles that encase miRNA and proteins. Here, we demonstrate that SVZ NSCs generate and release EVs. Neonatal electroporated fluorescent EV fusion proteins were released by NSCs and subsequently cleared from the SVZ. EVs were preferentially targeted to microglia. Small RNA sequencing identified miRNAs within the EVs that regulate microglia physiology and morphology. EVs induced a transition to a CD11b/Iba1 non-stellate microglial morphology. The transition accompanied a microglial transcriptional state characterized by Let-7-regulated cytokine release and a negative feedback loop that controlled NSC proliferation. These findings implicate an NSC-EV-microglia axis and provide insight to normal and pathophysiological brain development.

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Teaser

Morton et al. demonstrate that neonatal subventricular zone neural stem cells release extracellular vesicles. A combination of primary stem cell culture, in vivo genetic manipulations, next-generation small RNA sequencing, and neonatal extracellular vesicle transplantations reveal that extracellular vesicles are transferred to and regulate microglia in the neonatal brain.


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