Ultrasensitive competitive method-based electrochemiluminescence immunosensor for diethylstilbestrol detection based on Ru(bpy)32+ as luminophor encapsulated in metal–organic frameworks UiO-67

Publication date: 1 July 2018
Source:Biosensors and Bioelectronics, Volume 110
Author(s): Xue Dong, Guanhui Zhao, Li Liu, Xuan Li, Qin Wei, Wei Cao
In this work, Ru(bpy)3²⁺ encapsulated in metal–organic frameworks (MOFs) UiO-67 (Ru(bpy)3²⁺/UiO-67) as luminophor was easily prepared and firstly applied in constructing an electrochemiluminescence (ECL) immunosensor to efficiently estimate diethylstilbestrol (DES). The competitive method-based ECL immunosensor platform was fabricated by amino-silicon dioxide which possesses large surface area. The poriness of UiO-67 was splendid so that Ru(bpy)3²⁺ could be easily encapsulated. Ru(bpy)3²⁺/UiO-67 with excellent ECL luminescence signal existed large specific surface area for easily labeled with antibodies. DES competed with bovine serum albumin-diethylstilbestrol (BSA-DES) for binding to antibody-specific sites in the constructed immunosensor. However DES was micromolecule, which was easier to bond to antibodies than BSA-DES. The ECL signal was gradually decreases with the increase of the concentration of DES. Under optimal conditions, the proposed immunosensor exhibited a wide linear range from 0.01 pg mL⁻¹ to 50 ng mL⁻¹ with a low detetion limit of 3.27 fg mL⁻¹ (S/N = 3). The novel fabricated immunosensor with interference immunity and high stability may cause an attractive approach for the other targets determination.



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