A photostable fluorescent probe for long-time imagining of lysosome in cell and nematode

Publication date: 1 October 2018
Source:Talanta, Volume 188
Author(s): Changjian Li, Yueqi Wang, Saipeng Huang, Xiaojie Zhang, Xiaoxuan Kang, Yanchi Sun, Ziyuan Hu, Lu Han, Libo Du, Yang Liu
Lysosome fluorescent imaging has been widely used in the field of biological staining and diagnostics, which plays a key role in understanding intracellular metabolism and various physiological processes. However, for most currently used small-molecule lysotrackers, the photostability is often unsatisfactory when used for long-term and real-time visualization of lysosomal dynamics. Herein, we reported a new lysosome-targetable photostable fluorescent probe (i.e. MPL-NPA), and results showed that MPL-NAP possesses superior photostability, appreciable tolerance to pH change, low cytotoxicity and high lysosome targeting ability. These findings confirm that MPL-NAP is a well-suited imaging agent for targeting lysosome and enables long-term and real-time monitor of lysosome morphological changes under physiological processes.

Graphical abstract

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