Silica – Boronate affinity material for quick enrichment of intracellular nucleosides

Publication date: 1 May 2017
Source:Talanta, Volume 166
Author(s): Shuxia Wang, Huihui Li, Xiujuan Guan, Ting Cheng, Haixia Zhang
Boronic acid modified materials have been widely used to adsorb nucleosides, but their adsorption capacities require further improvement. Most cis-diol containing biomolecules are in very low abundance along with interfering components in real samples, and need to be enriched specially. In this study, we synthesize a kind of silica absorbent modified with boronic acid derivative, using amorphous silica as raw material and obtaining high adsorption capacity for adenosine. In addition, the adsorption equilibrium can be completed within 10s and 1min for the desorption. Finally, the material was successfully applied to enrich nucleosides from cells and the spiked recoveries were found between 82.21% and 118.9%. The results showed that the prepared adsorbent has potential to effectively enrich cis-diol substances from cell samples.

Graphical abstract

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