A medically relevant capsular polysaccharide in Acinetobacter baumannii is a potential vaccine candidate

Publication date: Available online 9 February 2017
Source:Vaccine
Author(s): Feng-Ling Yang, Tze-Chi Lou, Shu-Chen Kuo, Wan-Ling Wu, Jeffy Chern, Yi-Tzu Lee, Shui-Tsung Chen, Wei Zou, Nien-Tsung Lin, Shih-Hsiung Wu
Concerns of Acinetobacter baumannii infection have increased due to the emergence of multi-drug resistance. In the present study, we determined the capsular polysaccharide (CPS) structure of A. baumannii SK44, a clinical isolate from Taiwan, to consist of pentasaccharide repeats. We found that CPS-induced antibody provided 55% protection against challenge in an animal model. The CPS-specific antibody reacted with the surface components of about 62% clinical isolates (342/554 strains) from cross-sectional and longitudinal studies by dot-immunoassay. Pulsed-field gel electrophoresis of positive strains showed the antibody covered different clonalites of A. baumannii clinical isolates. Meanwhile, using the CPS antibody as a probe, we found a number of outer membrane proteins bound to the antibody, including OmpA/motB, TonB-dependent receptor, and Omp38, indicating their association with CPS. These results might lead to the use of the capsular polysaccharide as a vaccine to prevent A. baumannii infection.



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