Synthesis, hybridization and fluorescence properties of a 2′-C-pyrene-triazole modified arabino-uridine nucleotide

Publication date: Available online 9 February 2017
Source:Bioorganic & Medicinal Chemistry
Author(s): Pawan Kumar, Pawan K. Sharma, Poul Nielsen
A new pyrene-modified nucleotide monomer is introduced, wherein pyrene is attached to the 2′-position of arabino-uridine through a triazolemethyl linker. When the monomer is introduced into oligonucleotides, very stable DNA duplexes and three way junctions are obtained. An oligonucleotide featuring two modifications in the center shows four-fold increase in the intensity of the pyrene excimer signal on hybridization with an RNA target but not to a DNA target. The new nucleotide monomer has potential in DNA invader probes as well as in RNA targeting and detection.

Graphical abstract

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