Lipid Quantitation and Metabolomics Data from Vitamin E-Deficient and -Sufficient Zebrafish Embryos from 0 to 120 Hours-Post-Fertilization

Publication date: Available online 21 February 2017
Source:Data in Brief
Author(s): Melissa McDougall, Jaewoo Choi, Hye–Kyeong Kim, Gerd Bobe, J. Frederik Stevens, Enrique Cadenas, Robert Tanguay, Maret G. Traber
The data herein is in support of our research article by McDougal et al., Lethal Dysregulation of Energy Metabolism During Embryonic Vitamin E Deficiency [1], in which we used our zebrafish model of embryonic vitamin E (VitE) deficiency to study the consequences of VitE deficiency during development. Adult 5D wild-type zebrafish (Danio rerio), fed defined diets without (E–) or with VitE (E+, 500mg RRR-α-tocopheryl acetate/kg diet), were spawned to obtain E– and E+ embryos and evaluated using metabolomics and specific lipid analyses (each measure at 24, 48, 72, 120 hours-post-fertilization, hpf), neurobehavioral development (locomotor responses at 96 hpf), and rescue strategies. Rescues were attempted using micro-injection into the yolksac using VitE (as a phospholipid emulsion containing d6-α-tocopherol at 0 hpf) or D-glucose (in saline at 24 hpf).



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