l-Carnitine ameliorates knee lesions in mono-iodoacetate induced osteoarthritis in rats

Publication date: March 2017
Source:Alexandria Journal of Medicine, Volume 53, Issue 1
Author(s): Samia Mohamed Ali, Amr Abdel Kader Okda, Iman Samy Dessouky, Wafaa Ahmed Hewedy, Noha Mahmoud Zahran, Butheina Abdul-wally Alamrani
ObjectiveTo evaluate the chondroprotective effect of l-carnitine in relation to glucosamine sulfate and in an experimental model of osteoarthritis (OA).Materials and methodsThirty-two adult male Wister albino rats weighing 150–210g were assigned randomly into 4 groups: 8 rats in each group, group I (control group), group II (MIA induced OA group), group III (MIA induced OA+glucosamine sulfate treated group), and group IV (MIA induced OA+l-carnitine treated group). Weight, knee diameter, and knee bend score were recorded on days 0, 1, 7, 14 and 28. On day 28 all animals were sacrificed. Synovial fluid of left knee was collected, and the interleukin-1β (IL-1β), Cartilage oligomeric matrix protein (COMP) and matrix metalloproteinase-13 (MMP-13) levels were measured by ELISA. The knee joints were removed and stained with H&E for histological evaluation.ResultsThe pathological abnormalities attributed to MIA induced arthritis was dramatically lowered in rats treated with glucosamine or l-carnitine. Synovial fluid levels of IL-1β, COMP and MMP-13 were increased in OA group, and significantly reduced with glucosamine or l-carnitine treated groups.Conclusionl-Carnitine has a potential chondroprotective effect in this animal model of OA.



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