microRNA 183 family profiles in pheochromocytomas are related to clinical parameters and SDHB expression

Publication date: Available online 12 April 2017
Source:Human Pathology
Author(s): Suja Pillai, Chung Y. Lo, Victor Liew, Minella Lalloz, Robert A Smith, Vinod Gopalan, Alfred King-Yin Lam
This study aims to examine the expression profiles of the miR-183 cluster (miR-96/182/183) in pheochromocytoma. Pheochromocytoma tissues were prospectively collected from 50 patients with pheochromocytoma. Expression of miR-183 cluster members and SDHB protein expression were analysed in these tissues by quantitative real-time polymerase chain reaction and immunohistochemistry respectively. The expression of miR-183 cluster members in pheochromocytomas was correlated with the clinical and pathological parameters of these patients. The expression levels of miR-183 cluster members were predominantly downregulated or deleted in pheochromocytoma. Low expression or deletion of miR-96 was predominantly noted in younger age patients with pheochromocytoma (<50years, P=.01). Female patients in the study group showed marked deletion of miR-182 (P=.05). Deletion of the cluster was also associated with SDHB protein expression in pheochromocytoma. Moreover, patients with low miR-183 cluster expression had a slightly better survival rate when compared to patients with high expression. To conclude, the findings indicate a role for miR-183 cluster members in the pathogenesis and clinical progression of pheochromocytoma.



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