Study on the organization of cellular elements in the granulomatous lesion caused by chromoblastomycosis

ABSTRACT

Background

Chromoblastomycosis is a deep fungal infection characterized by a complex cellular granuloma. The aim of this study was to analyze the arrangement of cells responsible for the granuloma configuration of this disease by semiquantification of the cellular components of chromoblastomycosis skin biopsies.

Methods

The cells of cutaneous biopsies slides from 100 patients with untreated chromoblastomycosis were stained with hematoxylin-eosin and the granuloma cells were evaluated by microscopic examination of the elements of each granuloma and semiquantified the number of cells through its expressivity in crosses and the histopathological variables. Their presences were coded in degrees of intensity and classified in two categories: low expression and high expression. The cells that constitute the granulomas were separated into three groups: A, B and C.

Results

The chromoblastomycotic granuloma analysed by this semiquantification of its cellular components showed that there was high expression of the elements setting up a mixed organized mycotic granuloma. It was observed that mononuclear phagocytic system (A), polymorphonuclear phagocytic system (B) and lymphoplasmocytic infiltrate (C) were located around the fungus.

Conclusions

The results indicated that the granuloma present in the cutaneous lesion of chromoblastomycosis is the mixed organized mycotic granuloma with high expression of the cellular components.

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