Highly fluorescent nitrogen-doped carbon dots derived from Phyllanthus acidus utilized as a fluorescent probe for label-free selective detection of Fe3+ ions, live cell imaging and fluorescent ink

Publication date: 15 January 2018
Source:Biosensors and Bioelectronics, Volume 99
Author(s): Raji Atchudan, Thomas Nesakumar Jebakumar Immanuel Edison, Kanikkai Raja Aseer, Suguna Perumal, Namachivayam Karthik, Yong Rok Lee
A facile, economical and one-step hydrothermal method is used to synthesize highly durable fluorescent nitrogen-doped carbon dots (FNCDs) by utilizing Phyllanthus acidus (P. acidus) and aqueous ammonia as the carbon and nitrogen sources, respectively. The synthesized FNCDs have an average size of 4.5±1nm and showed bright blue fluorescence under the irradiation of UV-light at an excitation wavelength of 365nm. It exhibits a quantum yield (QY) of 14% at an excitation wavelength of 350nm with maximum emission at 420nm. X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscopy characterizations clearly showed the formation of FNCDs that predominantly consists of nitrogen and hydroxyl groups which can provide more adsorption sites. In addition, the above study reveals the successful bonding of nitrogen with carbon (C–N) in the FNCDs. The synthesized FNCDs with high QY can be used as efficient fluorescent probes for the detection of Fe³⁺. Based on the linear relationship between normalized fluorescence intensity and concentration of Fe³⁺ ions, the prepared FNCDs can be used for label-free sensitive and selective detection of Fe³⁺ ions in a wide concentration range of 2–25μM with a detection limit of 0.9μM. The present study proves that synthesized FNCDs has durable fluorescence, soluble in water very well and thus act as a promising candidate for the diverse applications such as label-free sensitive and selective detection of Fe³⁺, fluorescent ink and cellular imaging with good biocompatibility and low cytotoxicity.

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