A colorimetric evaluation of the effect of bacterial contamination on teeth stained with blood in vitro; evaluation of the efficacy of two different bleaching regimes

Abstract

Background

Tooth discolouration could occur due to bacterial contamination in traumatised teeth. Hydrogen peroxide is the commonly used bleaching agent. However, due to concerns over safety, alternative-bleaching regimes such as sodium perborate (S) and thiourea-hydrogen peroxide (T) have been investigated.

Methodology

Apices resected and pulp extirpated 99 premolars were divided into two groups. Group 1 and 2 was injected with blood and blood/bacteria, stored anaerobically for 35 days. The two groups were treated by bleaching with water, S or T. Teeth were rebleached after 7 days. Colourimetric evaluation was assessed using digital imaging, CasMatch standardization and CIE L*a*b colour system at Pre-operatively, 35 days of staining and 7 and 14 of bleaching. A linear mixed model with fixed effects of time, group and bleach was used to examine colour difference.

Results

Blood stained teeth were significantly redder and darker on day 35 compared to blood/bacteria stained teeth. After bleaching, blood stained teeth retained significant redness compared to blood/bacteria stained teeth using either S or T .T produced a significantly whiter shade in both the groups after 14 days.

Conclusions

Blood stained teeth were significantly darker and red compared to blood/bacteria stained teeth. T bleaching regime was more effective than S.

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