Publication date: 1 May 2018
Source:Biosensors and Bioelectronics, Volume 104
Author(s): Aigui Xu, Long Chao, Hongbo Xiao, Yuyun Sui, Jia Liu, Qingji Xie, Shouzhuo Yao
We report an ultrasensitive electrochemical sensor for Hg2+ detection, on the basis of two Hg2+-specific oligonucleotide probes (a thiolated capture probe and a biotinated signal probe), "terminal" signal amplification of alkaline phosphatase catalyzed deposition of silver and in situ microliter-droplet anodic stripping voltammetry (ASV). The thiolated capture probe is immobilized on an Au-plated glassy carbon electrode, and the biotinated signal probe is then attached to the electrode surface through the thymine-Hg2+-thymine interaction in the presence of Hg2+. A streptavidin-alkaline phosphatase (ALP) composite is then immobilized on the electrode surface via biotin–streptavidin interaction. The immobilized ALP can catalyze the hydrolyzation of ascorbic acid 2-phosphate trisodium salt to generate ascorbic acid, which can reduce AgNO3 to form silver deposit on the modified electrode. Quantitative analysis of Hg2+ is conducted through microliter-droplet ASV of silver after its simultaneous chemical dissolution and cathodic preconcentration on the modified electrode. The sensor can linearly respond to the common logarithm of Hg2+ concentration from 0.1nM to 250μM with a limit of detection of 0.01nM (2 ppt, S/N = 3) and a sensitivity as high as 227μAdec−1.
http://ift.tt/2CXhN6z
Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,
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