Bone marrow miR-10a overexpression is associated with genetic events but not affects clinical outcome in acute myeloid leukemia

Publication date: Available online 5 December 2017
Source:Pathology - Research and Practice
Author(s): Ting-juan Zhang, Hong Guo, Jing-dong Zhou, Xi-xi Li, Wei Zhang, Ji-chun Ma, Xiang-mei Wen, Xin-yu Yao, Jiang Lin, Jun Qian
BackgroundAccumulating studies have linked the disruptions of microRNA-10 (miR-10) to acute myeloid leukemia (AML) with NPM1 mutation. However, miR-10 expression and its clinical implication in AML remain poorly defined. Although a recent report showed high serum level of miR-10a was associated with adverse prognosis in AML, herein, we found bone marrow (BM) miR-10 overexpression was not a prognostic biomarker in AML.MethodsBM miR-10 expression was examined by real-time quantitative PCR in BM mononuclear cells in 115 de novo AML patients and 45 controls.ResultsBM miR-10 (miR-10a/b) expression was significantly up-regulated in AML patients, and was positively correlated with each other. Overexpression of miR-10a was associated with lower percentage of BM blasts, whereas miR-10b overexpression tended to correlate with higher percentage of BM blasts. Importantly, miR-10a overexpression was significantly associated with FAB-M3/t(15;17) subtypes and NPM1 mutation, meanwhile, overexpression of miR-10b was correlated with NPM1 and DNMT3A mutations. However, miR-10a/b overexpression was not associated with complete remission rate, and did not have an impact on both leukemia free survival and overall survival time in non-M3 AML patients without NPM1 mutation.ConclusionsBM miR-10 overexpression is associated with genetic events but not affects clinical outcome in AML.



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