Role of miR-1 expression in clear cell renal cell carcinoma (ccRCC): a bioinformatics study based on GEO, ArrayExpress microarrays and TCGA database

Publication date: Available online 2 December 2017
Source:Pathology - Research and Practice
Author(s): Hai-biao Yan, Jia-cheng Huang, You-rong Chen, Jian-ni Yao, Wei-ning Cen, Jia-yi Li, Yi-fan Jiang, Gang Chen, Sheng-hua Li
PurposeTo investigate the clinical value and potential molecular mechanisms of miR-1 in clear cell renal cell carcinoma (ccRCC).MethodsWe searched the Gene Expression Omnibus (GEO), ArrayExpress, several online publication databases and the Cancer Genome Atlas (TCGA). Continuous variable meta-analysis and diagnostic meta-analysis were conducted, both in Stata 14, to show the expression of miR-1 in ccRCC. Furthermore, we acquired the potential targets of miR-1 from datasets that transfected miR-1 into ccRCC cells, online prediction databases, differentially expressed genes from TCGA and literature. Subsequently bioinformatics analysis based on aforementioned selected target genes was conducted.ResultsThe combined effect was −0.92 with the 95% confidence interval (CI) of −1.08 to −0.77 based on fixed effect model (I2=81.3%, P<0.001). No publication bias was found in our investigation. Sensitivity analysis showed that GSE47582 and 2 TCGA studies might cause heterogeneity. After eliminating them, the combined effect was −0.47 (95%CI: −0.78, −0.16) with I2=18.3%. As for the diagnostic meta-analysis, the combined sensitivity and specificity were 0.90 (95%CI: 0.61, 0.98) and 0.63 (95%CI: 0.39, 0.82). The area under the curve (AUC) in the summarized receiver operating characteristic (SROC) curve was 0.83 (95%CI: 0.80, 0.86). No publication bias was found (P=0.15). We finally got 67 genes which were defined the promising target genes of miR-1 in ccRCC. The most three significant KEGG pathways based on the aforementioned genes were Complement and coagulation cascades, ECM-receptor interaction and Focal adhesion.ConclusionThe downregulation of miR-1 might play an important role in ccRCC by targeting its target genes.



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