Related Articles Simultaneous recording and marking of brain microstructures. J Neural Eng. 2020 Jun 30;: Authors: Ramadi KB, Dagdeviren C, Bhagchandani P, Nunez-Lopez C, Kim MJ, Langer RS, Graybiel A, Cima MJ Abstract The vast majority of techniques to study the physiology of the nervous system involve inserting probes into the brain for stimulation, recording, or sampling. Research is increasingly uncovering the fine microstructure of the...
Wed Jul 01, 2020 15:31
65 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: "Proc Natl Acad Sci U S A"[jour] These pubmed results were generated on 2020/07/01PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Wed Jul 01, 2020 15:28
Related Articles Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells. J Vis Exp. 2020 Jun 09;(160): Authors: Maliszewska-Olejniczak K, Dróżdż A, Waluś M, Dorosz M, Gryziński MA Abstract The purpose of the manuscript is to provide a step-by-step protocol for performing immunofluorescence microscopy to study the radiation-induced DNA damage response induced by neutron-gamma mixed-beam used in boron neutron...
Wed Jul 01, 2020 15:27
Related Articles A Preclinical Controlled Cortical Impact Model for Traumatic Hemorrhage Contusion and Neuroinflammation. J Vis Exp. 2020 Jun 10;(160): Authors: Lee HF, Chen CH, Chang CF Abstract Cerebral contusion is a severe medical problem affecting millions of people worldwide each year. There is an urgent need to understand the pathophysiological mechanism and to develop effective therapeutic strategy for this devastating neurological...
Wed Jul 01, 2020 15:27
Related Articles Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair. J Vis Exp. 2020 Jun 09;(160): Authors: Nakane T, Abulaiti M, Sasaki Y, Kowalski WJ, Keller BB, Masumoto H Abstract The current protocol describes methods to generate scalable, mesh-shaped engineered cardiac tissues (ECTs) composed of cardiovascular cells derived from human induced pluripotent stem cells (hiPSCs),...
Wed Jul 01, 2020 15:27
Related Articles Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells. J Vis Exp. 2020 Jun 13;(160): Authors: Lee J, Lee JE, Kim S, Kang D, Yoo HM Abstract This manuscript describes a protocol to evaluate cancer cell deaths in three dimensional (3D) spheroids of multicellular types of cancer cells using supernatants from Lactobacillus fermentum cell culture, considered...
Wed Jul 01, 2020 15:27
Related Articles Semi-Minimal Invasive Method to Induce Myocardial Infarction in Rats and the Assessment of Cardiac Function by an Isolated Working Heart System. J Vis Exp. 2020 Jun 11;(160): Authors: Pilz PM, Lang M, Hamza O, Szabo PL, Inci M, Kramer AM, Koch M, Huber J, Podesser BK, Kiss A Abstract Myocardial infarction (MI) remains the main contributor to morbidity and mortality worldwide. Therefore, research on this topic is mandatory....
Wed Jul 01, 2020 15:27
Related Articles Investigation of Xenobiotics Metabolism In Salix alba Leaves via Mass Spectrometry Imaging. J Vis Exp. 2020 Jun 15;(160): Authors: Villette C, Maurer L, Heintz D Abstract The method presented uses mass spectrometry imaging (MSI) to establish the metabolic profile of S. alba leaves when exposed to xenobiotics. Using a non-targeted approach, plant metabolites and xenobiotics of interest are identified and localized in plant...
Wed Jul 01, 2020 15:27
Related Articles Isolation of L-form Bacteria from Urine using Filtration Method. J Vis Exp. 2020 Jun 13;(160): Authors: Davison F, Chapman J, Mickiewicz K Abstract Transition of bacteria to the L-form state is thought to play a possible role in immune evasion and bacterial persistence during treatment with cell wall-targeting antibiotics. However, isolation and handling of L-form bacteria is challenging, mainly due to their high sensitivity...
Wed Jul 01, 2020 15:27
Related Articles In Situ Measurement of Vacuum Window Birefringence using 25Mg+ Fluorescence. J Vis Exp. 2020 Jun 13;(160): Authors: Yuan WH, Liu HL, Wei WZ, Ma ZY, Hao P, Deng Z, Deng K, Zhang J, Lu ZH Abstract Accurate control of the polarization states of laser light is important in precision measurement experiments. In experiments involving the use of a vacuum environment, the stress-induced birefringence effect of the vacuum windows...
Wed Jul 01, 2020 15:27
Related Articles Assessing the Age-Specific Phagocytic Ability of Adult Drosophila melanogaster Hemocytes using an In Vivo Phagocytosis Assay. J Vis Exp. 2020 Jun 11;(160): Authors: Campbell SM, Starz-Gaiano M, Leips J Abstract Phagocytosis is an essential function of the innate immune response. This process is carried out by phagocytic hemocytes whose primary function is to recognize a wide range of particles and destroy microbial pathogens....
Wed Jul 01, 2020 15:27
Related Articles Culture of Neurospheres Derived from the Neurogenic Niches in Adult Prairie Voles. J Vis Exp. 2020 Jun 10;(160): Authors: Ávila-González D, Young LJ, Camacho F, Paredes RG, Díaz NF, Portillo W Abstract Neurospheres are primary cell aggregates that comprise neural stem cells and progenitor cells. These 3D structures are an excellent tool to determine the differentiation and proliferation potential of neural stem cells, as...
Wed Jul 01, 2020 15:27
Related Articles Manufacturing, Control, and Performance Evaluation of a Gecko-Inspired Soft Robot. J Vis Exp. 2020 Jun 10;(160): Authors: Schiller L, Seibel A, Schlattmann J Abstract This protocol presents a method for manufacturing, control, and evaluation of the performance of a soft robot that can climb inclined flat surfaces with slopes of up to 84°. The manufacturing method is valid for the fast pneunet bending actuators in general...
Wed Jul 01, 2020 15:27
Related Articles Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos. J Vis Exp. 2020 Jun 12;(160): Authors: Logsdon DM, Kile RA, Schoolcraft WB, Krisher RL, Yuan Y Abstract Human implantation, the apposition and adhesion to the uterine surface epithelia and subsequent invasion of the blastocyst into the maternal decidua, is a critical yet enigmatic biological event that has been historically difficult to...
Wed Jul 01, 2020 15:27
Related Articles A Patient-Derived Xenograft Model for Venous Malformation. J Vis Exp. 2020 Jun 15;(160): Authors: Schrenk S, Goines J, Boscolo E Abstract Venous malformation (VM) is a vascular anomaly that arises from impaired development of the venous network resulting in dilated and often dysfunctional veins. The purpose of this article is to carefully describe the establishment of a murine xenograft model that mimics human VM and is...
Wed Jul 01, 2020 15:27
Related Articles Tracking Rats in Operant Conditioning Chambers Using a Versatile Homemade Video Camera and DeepLabCut. J Vis Exp. 2020 Jun 15;(160): Authors: Clemensson EKH, Abbaszadeh M, Fanni S, Espa E, Cenci MA Abstract Operant conditioning chambers are used to perform a wide range of behavioral tests in the field of neuroscience. The recorded data is typically based on the triggering of lever and nose-poke sensors present inside the...
Wed Jul 01, 2020 15:27
Related Articles Continuous Video Electroencephalogram during Hypoxia-Ischemia in Neonatal Mice. J Vis Exp. 2020 Jun 11;(160): Authors: Wagley PK, Williamson J, Skwarzynska D, Kapur J, Burnsed J Abstract Hypoxia ischemia is the most common cause of neonatal seizures. Animal models are crucial for understanding the mechanisms and physiology underlying neonatal seizures and hypoxia ischemia. This manuscript describes a method for continuous...
Wed Jul 01, 2020 15:27
Related Articles Ex Vivo Myelination and Remyelination in Cerebellar Slice Cultures as a Quantitative Model for Developmental and Disease-Relevant Manipulations. J Vis Exp. 2020 Jun 12;(160): Authors: Shen K, Yuen TJ Abstract Studying myelination in vitro and in vivo poses numerous challenges. The differentiation of oligodendrocyte precursor cells (OPCs) in vitro, although scalable, does not recapitulate axonal myelination. OPC-neuron cocultures...
Wed Jul 01, 2020 15:27
Related Articles Ground-level Unmanned Aerial System Imagery Coupled with Spatially Balanced Sampling and Route Optimization to Monitor Rangeland Vegetation. J Vis Exp. 2020 Jun 14;(160): Authors: Curran MF, Hodza P, Cox SE, Lanning SG, Robertson BL, Robinson TJ, Stahl PD Abstract Rangeland ecosystems cover 3.6 billion hectares globally with 239 million hectares located in the United States. These ecosystems are critical for maintaining...
Wed Jul 01, 2020 15:27
Related Articles Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications. J Vis Exp. 2020 Jun 12;(160): Authors: Benitez GJ, Shinoda K Abstract Brown and beige fat are specialized adipose tissues that dissipate energy for thermogenesis by UCP1 (Uncoupling Protein-1)-dependent and independent pathways. Until recently, thermogenic adipocytes were considered a homogeneous population. However, recent studies have indicated that...
Wed Jul 01, 2020 15:27
Related Articles A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT. J Vis Exp. 2020 Jun 12;(160): Authors: Andronowski JM, Davis RA, Holyoke CW Abstract Bone is a dynamic and mechanically active tissue that changes in structure over the human lifespan. The products of the bone remodeling process have been studied substantially using traditional two-dimensional...
Wed Jul 01, 2020 15:27
Related Articles P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives. J Vis Exp. 2020 Jun 15;(160): Authors: Montefusco-Pereira CV, Horstmann JC, Ebensen T, Beisswenger C, Bals R, Guzmán CA, Schneider-Daum N, Carvalho-Wodarz CS, Lehr CM Abstract fDrug research for the treatment of lung infections is progressing towards predictive in vitro...
Wed Jul 01, 2020 15:27
Related Articles Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis. J Vis Exp. 2020 Jun 10;(160): Authors: Chiarelli TJ, Grieshaber NA, Grieshaber SS Abstract The intracellular bacterial pathogen Chlamydia trachomatis undergoes a developmental cycle consisting of two morphologically discrete developmental forms. The non-replicative elementary body (EB) initiates infection of the host....
Wed Jul 01, 2020 15:27
Related Articles Biventricular Assessment of Cardiac Function and Pressure-Volume Loops by Closed-Chest Catheterization in Mice. J Vis Exp. 2020 Jun 15;(160): Authors: Potus F, Martin AY, Snetsinger B, Archer SL Abstract Assessment of cardiac function is essential to conduct cardiovascular and pulmonary-vascular preclinical research. Pressure-volume loops (PV loops) generated by recording both pressure and volume during cardiac catheterization...
Wed Jul 01, 2020 15:27
Related Articles Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method. J Vis Exp. 2020 Jun 14;(160): Authors: Veerapandian R, Paudyal A, Chang A, Vediyappan G Abstract Natural products derived from plants and microbes are a rich source of bioactive molecules. Prior to their use, the active molecules from complex extracts must be purified for downstream...
Wed Jul 01, 2020 15:27
Related Articles Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue. J Vis Exp. 2020 Jun 09;(160): Authors: Barwinska D, Ferkowicz MJ, Cheng YH, Winfree S, Dunn KW, Kelly KJ, Sutton TA, Rovin BH, Parikh SV, Phillips CL, Dagher PC, El-Achkar TM, Eadon MT, Kidney Precision Medicine Project Abstract Gene expression analysis of human kidney tissue is an important tool to understand homeostasis and...
Wed Jul 01, 2020 15:27
Related Articles Noninvasive Electrocardiography in the Perinatal Mouse. J Vis Exp. 2020 Jun 12;(160): Authors: Fitzsimons LA, Brewer VL, Forrester J, Moran AM, Tucker KL Abstract Electrocardiography (ECG) has long been relied upon as an effective and reliable method of assessing cardiovascular (and cardiopulmonary) function in both human and animal models of disease. Individual heart rate, rhythm, and regularity, combined with quantitative...
Wed Jul 01, 2020 15:27
Related Articles Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations. J Vis Exp. 2020 Jun 14;(160): Authors: Mateos-White I, Fabra-Beser J, de Agustín-Durán D, Gil-Sanz C Abstract In utero electroporation is an in vivo DNA transfer technique extensively used to study the molecular and cellular mechanisms underlying mammalian corticogenesis. This procedure takes advantage of the brain ventricles...
Wed Jul 01, 2020 15:27
Related Articles A Benchtop Approach to the Location Specific Blood Brain Barrier Opening using Focused Ultrasound in a Rat Model. J Vis Exp. 2020 Jun 13;(160): Authors: Rich M, Whitsitt Q, Lubin F, Bolding M Abstract Stereotaxic surgery is the gold standard for localized drug and gene delivery to the rodent brain. This technique has many advantages over systemic delivery including precise localization to a target brain region and reduction...
Wed Jul 01, 2020 15:27
Related Articles Using a Virtual Reality Walking Simulator to Investigate Pedestrian Behavior. J Vis Exp. 2020 Jun 09;(160): Authors: Chung HC, Kim SH, Choi G, Kim JW, Choi MY, Li H Abstract To cross a road successfully, individuals must coordinate their movements with moving vehicles. This paper describes use of a walking simulator in which people walk on a treadmill to intercept gaps between two moving vehicles in an immersive virtual...
Wed Jul 01, 2020 15:27
Related Articles High-Throughput Assays of Critical Thermal Limits in Insects. J Vis Exp. 2020 Jun 15;(160): Authors: Awde DN, Fowler TE, Pérez-Gálvez F, Garcia MJ, Teets NM Abstract Upper and lower thermal limits of plants and animals are important predictors of their performance, survival, and geographic distributions, and are essential for predicting responses to climate change. This work describes two high-throughput protocols for measuring...
Wed Jul 01, 2020 15:27
Related Articles Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses. J Vis Exp. 2020 Jun 14;(160): Authors: Laval K, Maturana CJ, Enquist LW Abstract This protocol describes a footpad inoculation model used to study the initiation and development of neuroinflammatory responses during alphaherpesvirus infection in mice. As alphaherpesviruses are main invaders of the peripheral nervous system (PNS), this model...
Wed Jul 01, 2020 15:27
Related Articles Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining. J Vis Exp. 2020 Jun 13;(160): Authors: Silva TP, Fernandes TG, Nogueira DES, Rodrigues CAV, Bekman EP, Hashimura Y, Jung S, Lee B, Carmo-Fonseca M, Cabral JMS Abstract The cerebellum plays a critical role in the maintenance of balance and motor coordination, and a functional defect in different cerebellar...
Wed Jul 01, 2020 15:27
Related Articles Longitudinal In Vivo Imaging and Quantification of Human Pancreatic Islet Grafting and Contributing Host Cells in the Anterior Eye Chamber. J Vis Exp. 2020 Jun 11;(160): Authors: Nilsson J, Holmberg D, Schmidt-Christensen A Abstract Imaging beta cells is a key step towards understanding islet transplantation. Although different imaging platforms for the recording of beta cell biology have been developed and utilized in...
Wed Jul 01, 2020 15:27
Related Articles Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A). J Vis Exp. 2020 Jun 10;(160): Authors: Niikura Y, Fang L, Kitagawa R, Li P, Xi Y, You J, Guo Y, Kitagawa K Abstract Studying the structure and the dynamics of kinetochores and centromeres is important in understanding chromosomal instability (CIN) and cancer progression. How the chromosomal location and function of a centromere (i.e.,...
Wed Jul 01, 2020 15:27
Related Articles CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis. J Vis Exp. 2020 Jun 09;(160): Authors: Wilson AM, Wingfield BD Abstract The CRISPR-Cas9 genome editing system is a molecular tool that can be used to introduce precise changes into the genomes of model and non-model species alike. This technology can be used for a variety of genome editing approaches, from gene knockouts and knockins...
Wed Jul 01, 2020 15:27
Related Articles Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae. J Vis Exp. 2020 Jun 11;(160): Authors: Poças GM, Domingos PM, Mirth CK Abstract Foraging and feeding behaviors allow animals to access sources of energy and nutrients essential for their development, health, and fitness. Investigating the neuronal regulation of these behaviors is essential for the understanding of the physiological...
Wed Jul 01, 2020 15:27
Related Articles Simultaneous Isolation and Culture of Atrial Myocytes, Ventricular Myocytes, and Non-Myocytes from an Adult Mouse Heart. J Vis Exp. 2020 Jun 14;(160): Authors: Blackwood EA, Bilal AS, Azizi K, Sarakki A, Glembotski CC Abstract The isolation and culturing of cardiac myocytes from mice has been essential for furthering the understanding of cardiac physiology and pathophysiology. While isolating myocytes from neonatal mouse...
Wed Jul 01, 2020 15:27
Related Articles In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2. J Vis Exp. 2020 Jun 13;(160): Authors: Pigazzini ML, Kirstein J Abstract Proteins are synthesized and degraded constantly within a cell to maintain homeostasis. Being able to monitor the degradation of a protein of interest is key to understanding not only its life cycle, but also to uncover imbalances in the proteostasis network....
Wed Jul 01, 2020 15:27
Related Articles Measuring the Confluence of iPSCs using an Automated Imaging System. J Vis Exp. 2020 Jun 10;(160): Authors: Magliocca V, Vinci M, Persichini T, Locatelli F, Tartaglia M, Compagnucci C Abstract This study focuses on understanding how growing iPSCs on different ECM coating substrates can affect cell confluence. A protocol to assess iPSC confluence in real time has been established without the need to count cells in single...
Wed Jul 01, 2020 15:27
Related Articles Investigating the Relationship between Sea Surface Chlorophyll and Major Features of the South China Sea with Satellite Information. J Vis Exp. 2020 Jun 13;(160): Authors: Chen HH, Tang R, Zhang HR, Yu Y, Wang Y Abstract Satellite observations offer a great approach to investigate the features of major marine parameters, including sea surface chlorophyll (CHL), sea surface temperature (SST), sea surface height (SSH), and...
Wed Jul 01, 2020 15:27
Related Articles The Adventures of Fundi Intervention Based on the Cognitive and Emotional Processing in Attention Deficit Hyperactive Disorder Patients. J Vis Exp. 2020 Jun 12;(160): Authors: Mayoral-Rodríguez S, Pérez-Álvarez F, Timoneda-Gallart C, Muñoz-Cuatrecasas A Abstract Many children with attention deficit hyperactive disorder (ADHD) are known to have executive dysfunction, which weakens their abilities to learn and behave in daily...
Wed Jul 01, 2020 15:27
Related Articles A Drosophila Model to Study Wound-induced Polyploidization. J Vis Exp. 2020 Jun 09;(160): Authors: Bailey EC, Dehn AS, Gjelsvik KJ, Besen-McNally R, Losick VP Abstract Polyploidy is a frequent phenomenon whose impact on organismal health and disease is still poorly understood. A cell is defined as polyploid if it contains more than the diploid copy of its chromosomes, which is a result of endoreplication or cell fusion....
Wed Jul 01, 2020 15:27
Related Articles Use of Real-Time Functional Magnetic Resonance Imaging-Based Neurofeedback to Downregulate Insular Cortex in Nicotine-Addicted Smokers. J Vis Exp. 2020 Jun 10;(160): Authors: Rana M, Ruiz S, Corzo AS, Muehleck A, Eck S, Salinas C, Zamorano F, Silva C, Rea M, Batra A, Birbaumer N, Sitaram R Abstract It has been more than a decade since the first functional magnetic resonance imaging (fMRI)-based neurofeedback approach was...
Wed Jul 01, 2020 15:27
Related Articles Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue. J Vis Exp. 2020 Jun 14;(160): Authors: Smith N, Bekaddour N, Leboulanger N, Richard Y, Herbeuval JP Abstract Studying isolated cells from mucosa-associated lymphoid tissues (MALT) allows understanding of immune cells response in pathologies involving mucosal immunity, because they can model host-pathogen...
Wed Jul 01, 2020 15:27
Related Articles In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide. J Vis Exp. 2020 Jun 11;(160): Authors: Feldman KS, Zahid M Abstract Since the initial description of protein transduction domains, also known as cell penetrating peptides, over 25 years ago, there has been intense interest in developing these peptides, especially cell-specific ones, as novel vectors for delivering diagnostic and therapeutic materials....
Wed Jul 01, 2020 15:27
Related Articles Delivery of Modified mRNA in a Myocardial Infarction Mouse Model. J Vis Exp. 2020 Jun 11;(160): Authors: Kaur K, Sultana N, Hadas Y, Magadum A, Sharkar MTK, Chepurko E, Zangi L Abstract Myocardial infarction (MI) is a leading cause of morbidity and mortality in the Western world. In the past decade, gene therapy has become a promising treatment option for heart disease, owing to its efficiency and exceptional therapeutic...
Wed Jul 01, 2020 15:27
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